EINLEITUNG

Two tube multiplex Real-Time PCR for detection of norovirus G1/G2, astrovirus, rotavirus and adenovirus – including internal control.

TESTPRINZIP UND -DURCHFüHRUNG

• Extraction of nucleic acid from stool samples of human origin
• High reliability: internal control (IC), which is introduced into the lysis buffer at the extraction stage of EACH specimen
• High safety: includes positive and negative controls for all pathogens
• Positive control pool: ONE positive pool for all pathogens to keep enzyme costs low
• Amplification and simultaneous detection of up to three pathogens in one tube

PACKUNGSINHALT

• 2 Optimized Primer and probe mixes
• Positive pool
• Negative control
• Internal control for confirming sample extraction

TECHNISCHE VORAUSSETZUNGEN

• The test is available for use with the ABI 7500, Rotor-Gene 6000, Bio-Rad CFX96 and the LightCycler480.
• This assay has been fully validated on an ABI7500 with the AgPath-ID™ One-Step RT-PCR Kit (Ambion, cat # AM1005).

HANDELSFORM