INTRODUCTION

Real-Time PCR for detection AND quantification of cytomegalovirus (CMV) – including internal control

TESTPRINCIPLE AND PROCEDURE

• Extraction of CMV DNA from plasma from EDTA/citrated blood
• High reliability: internal control (IC), which is introduced into the lysis buffer at the extraction stage of EACH specimen
• High safety: includes positive and negative controls for all pathogens
• Amplification and simultaneous detection
• High accuracy: a standard curve is generated by supplied quantification standards (QS1; QS2; QS3)

CONTENTS OF THE TEST

• Optimized Primer and probe mix
• Positive and negative control
• Internal control for confirming sample extraction
• Three quantification standards

TECHNICAL REQUIREMENTS

• The test is available for use with the ABI 7500, Rotor-Gene 6000, Bio-Rad CFX96 and the LightCycler480.
• This assay has been fully validated on an ABI7500 with the AgPath-ID™ One-Step RT-PCR Kit (Ambion, cat # AM1005).

COMMERCIAL PRODUCT