INTRODUCTION

Multiplex PCR for detection of influenza A (FluA) and B (FluB) and the swine-lineage influenza A virus subtype H1N1 (A(H1N1)swl) and internal control.

TESTPRINCIPLE AND PROCEDURE

• Extraction of total nucleic acid from respiratory samples (throat/nasal swabs, bronchoalveolar lavage, sputum) of human origin.
• High reliability: internal control (IC), which is introduced into the lysis buffer at the extraction stage of EACH specimen
• High safety: includes positive and negative controls for all pathogens
• Amplification and simultaneous detection of up to four pathogens in one tube

CONTENTS OF THE TEST

• Optimized primer and probe mix
• Positive and negative control
• Internal control for confirming sample extraction

TECHNICAL REQUIREMENTS

• The test is available for use with the ABI 7500, Rotor-Gene 6000, Bio-Rad CFX96 and the LightCycler480.
• This assay has been fully validated on an ABI7500 with the AgPath-ID™ One-Step RT-PCR Kit (Ambion, cat # AM1005).

COMMERCIAL PRODUCT