INTRODUCTION

Five tube multiplex Real-Time PCR for detection of 21 respiratory pathogens: influenza A, influenza A (H1N1), influenza B, coronaviruses NL63, 229E, OC43 and HKU1, parainfluenza 1, 2, 3 and 4, rhinovirus, respiratory syncytial viruses A and B, adenovirus, enterovirus, parechovirus, bocavirus and Mycoplasma pneumoniae.

See also FTD Respiratory 21 PLUS.

TESTPRINCIPLE AND PROCEDURE

• Extraction of total nucleic acid from respiratory samples (throat/nasal swabs, bronchoalveolar lavage, sputum) of human origin. In some cases other sample types may be tested including post mortem material such as heart and lung.
• High reliability: internal control (IC), which is introduced into the lysis buffer at the extraction stage of EACH specimen
• High safety: includes positive and negative controls for all pathogens
• Positive control pool: ONE positive pool for all pathogens to keep enzyme costs low
• Amplification and simultaneous detection of up to four pathogens in one tube

CONTENTS OF THE TEST

• 5-6 Optimized primer and probe mixes
• Positive pool
• Negative control
• Internal control

TECHNICAL REQUIREMENTS

• The test is available for use with the ABI 7500, Rotor-Gene 6000, Bio-Rad CFX96 and the LightCycler480.
• This assay has been fully validated on an ABI7500 with the AgPath-ID™ One-Step RT-PCR Kit (Ambion, cat # AM1005).

COMMERCIAL PRODUCT