LUMINEX^® xMAP^® TECHNOLOGY

Analyzer
The xMAP^® technology, developed by the Luminex^® corporation, provides an innovative platform for the analysis of multiplex assay systems. The analysis system comprises a workstation (e.g. LX 200 or MAGPIX), an automated fluidic system, a computer, a monitor and software.

LX 200 System
The method used is based on flow cytometry. The microspheres are separated when injected into the measuring cell. Two lasers are focused on the particle beam in the measuring cell. A red laser identifies the colour code of the bead while the quantitative analysis is carried out by a second green laser. Classical MicroPlex beads as well as the new magnetic MagPlex beads can be used.

MAGPIX System
Magnetic beads (MagPlex) are used in the MAGPIX system allowing a straight forward automation. A CCD camera detects the twodimensional magnetically fixed beads in the red and green light spectrum. MAGPIX is a compact and robust system with short startup and shut-down protocols.

Dynex DSX
The DSX is an open, fully automated analyzer for the complete sample preparation and processing of microtiter plate tests. The instrument is controlled through user-friendly Windows software, with which the user can program any separate assays. Due to the high flexibility and usability, the DSX supports daily routine work. The sample preparation can be carried out directly in a deep well plate.
The device can process four microtiter plates simultaneously and at the same time be continuously reloaded.

The DSX can be equipped with a magnetic plate for MagPlex beads.

Tecan Hydroflex
Automatic washing the plate after serum incubation and conjugate incubation on Tecan Hydroflex or Biotek ELx50 with magnetic disk.

SOFTWARE

The evaluation of the test recomBead systems takes place in two steps:
i) measuring of the beads by MAGPIX or LX 100/200 system with the Luminex^® xPONENT software and ii) test specific interpretation of the measurement with the MIRKOGEN recomQuant software.

xPONENT
The Luminex^® xPONENT software is a modular and flexible software pakage for the control of the MAGPIX and LX 100/200 systems. The software’s graphical user interface follows the typical assay workflow. Navigation wizards and automated routine operations such as startup, shutdown, calibration and performance verification, further enhance system usability and result in increased walkaway time. Different access levels allows the user to acquire samples, perform analysis, run system maintenance routines or to administrate the system.

recomQuant
The MIKROGEN specific test evaluation is done with the recomQuant software which accesses the raw data of the xPONENT software. recomQuant offers a high level of security: Lot data can be imported via download or barcode. In addition, lot data are connected to the shelf life of the test kits. The conjugate classes used are automatically detected and assigned.

Data import and export is simple and intuitive. The results are presented clearly.