recomLine ANA/ENA IgG
Line immunoassay using recombinant antigens to detect IgG autoantibodies in Connective Tissue Diseases (CTD).
Autoantibodies are immunglobulines which are directed against endogenous structures. In the case of CTD, the connective tissue is affected, which leads to inflammation, loss of elasticity and joint pain. According to their clinical symptoms, these diseases are assigned to the rheumatic disorders. The most frequent types of connective tissue diseases are:
- Systemic lupus erythematosus (SLE)
- Sjögren´s syndrom (SjS)
- Mixed connective tissue disease (MCTD)
- Progressive systemic scleroderma (PSS)
- Myositis
Autoantibodies are important diagnostic parameters with unclear pathogenetic significance, but of extraordinary relevance for differential diagnosis. The recomLine ANA/ENA IgG serves for the differentiation between rheumatic autoimmune diseases and other rheumatic diseases with similar symptoms.
Product advantages
- High sensitivity and specificity ensured by the use of recombinant antigens
- Fast and easy to use due to automation and software based evaluation
- One single approach to differentiate between the most frequent autoimmune collagenose diseases enabled by the use of 15 different antigens
- Highly reliable diagnostic of SLE using subunits of Sm and RNP antigens and high specific SLE markers
- Reliable diagnostic due to internal cut-off-control and IgG control
- Compatible with all other MIKROGEN line assays - reagents exchangeable
- CE label: The recomLine ANA/ENA IgG test meets the high standard of the EC directive 98/79/EC on in vitro diagnostic medical devices
Testprinciple and procedure
1st Incubation
A test strip loaded with autoimmune antigens is incubated with diluted serum or plasma in an incubation tray for 1 hour.
wash 3 times
2nd Incubation
Peroxidase conjugated anti-human antibodies (IgG specific) are added. Incubate for 45 minutes.
wash 3 times
Color reaction
Within 8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.
