recomWell EBV EA IgG
Enzyme immunoassay with antigens produced by recombinant techniques for the detection of IgG and IgM antibodies against Epstein-Barr Virus (EBV)
The Epstein-Barr virus, an ubiquitously occurring herpes virus, can cause the symptoms of infectious mononucleosis (Pfeiffer´s disease) on primary infection. Moreover, as a result of the lifelong persistence of this pathogen, reactivations can occur, especially in immuno-incompetent persons.
Due to the diversity of symptoms caused by EBV infection and their correspondence with the symptoms of other diseases, a secure EBV diagnosis is of great relevance for differential diagnostics. One of the main tasks in routine diagnosis is therefore the serological differentiation of a primary infection from a past infection and the exclusion of an EBV infection.
The recomWell EBV ELISA test systems are highly sensitive screening tests for detection of Epstein-Barr virus antibodies (IgG, IgM). Due to genetical engineering highly specific and characteristic EBV proteins can be used. A classification into the different stages of infection (primary infection, past infection) is possible due to the time-shifted appearance of antibodies against different EBV antigens.
Product advantages
- Reliable EBV screening
- Safe detection of past infections with the recomWell EBV EBNA IgG due to the very high specificity of the diagnostic EBV key antigen EBNA-1
- Detection of primary EBV infections already in the early phase due to the optimised antigen composition of the recomWell EBV VCA IgG and recomWell EBV IgM test systems
- No Rheumatoid factor treatment in the recomWell IgM test system necessary
- Recombinant antigens
- High Sensitivity and specificity
- Excellent discrimination between negative and positive results
- Identical procedure for IgG and IgM determination
- Easy to quantify
- Easy test procedure; automation possible
- Test procedure and reagents identical in all recomWell ELISA - reagents exchangeable
- Break-a-parts: Single sample examination possible
- CE label: The recomWell EBV test systems meet the high standard of the EC directive 98/79/EC on in vitro diagnostic medical devices
Testprinciple and procedure
Indirect sandwich test.
Recombinant antigens are bound to the solid phase.
1st Incubation
Add patient samples diluted 1:101 (sample: 10 µl of serum or plasma), incubate for 1 h at 37 °C.
Wash 4 times
2nd Incubation
Add peroxidase conjugated anti-human IgG or IgM antibodies (conjugate), incubate for 30 min at 37 °C.
Wash 4 times
Color reaction
Add ready-to-use TMB solution and incubate for 30 min at room temperature. Stop the substrate reaction with H3PO4 and measure the extinction at 450 nm.
