ampliCube MDR Panel 1-6
Real-Time PCR for the detection of transmittable resistance genes of multiresistant bacteria Multi Drug Resistance (MDR).
Multiresistant bacteria have become an emerging threat to healthcare systems all around the globe within the last years. A magnitude of pathogens contains transmittable resistance genes which allow them to survive the administration of formerly effective antibiotics. Due to those mechanisms modern medicine is slowly losing one of its most effective treatment methods.
In order to adequately meet this challenge and to sharpen the focus on the development of new antibiotic substances, the world health organization (WHO) has issued a list of multiresistant bacteria according to their relevance in 2017. WHO sees the highest potential threat in carbapenem-resistant Acinetobacter baumanii and Pseudomonas aeruginosa as well as Enterobacteriaceae which are resistant to carbapenems and 3rd generation cephalosporins.
The worldwide danger by carbapenem-resistant pathogens is based on the increasing incidence, the lack of safe and effective treatment alternatives as well as high mortality rates caused by resistant gram negative germs. Besides the development of novel substances, detection and analysis of resistance mechanisms are essential to overcome this global epidemic. This is important for acute treatment as well as for epidemiological surveillance.
Resistance to carbapenems can be acquired through various mechanisms. One of the most important ones is the production of carbapenemases and other ß-lactamases (ESBL) which inactivate antibiotic substances. The key to reduce mortality and hospital stays is the fastest possible detection of specific resistance mechanisms.
Carbapenemases or ß-lactamases can be roughly classified on the basis of their catalytical domain into metallo-ßlactamases and serine-ß-lactamases, which also contain further subsections. Relevant resistance genes of serineß-lactamases are e.g. KPC, CTX-M and OXA, common resistance genes of metallo-ß-lactamases are NDM, VIM and IMP. It is therefore crucial for the choice of an effective antibiotic (or a combination of several substances) to obtain a clear differentiation and analysis of the respective set of resistance genes.
Moreover, the increase of resistance against linezolid and vancomycin – two important reserve antibiotics for the treatment of infections with gram positive bacteria such as Staphylococcus and Enterococcus – becomes a life-threatening problem. A fast and precise analysis is again a key tool for a targeted antibiotic therapy. The detection of resistance genes via nucleic acid amplification technology (NAT) is a fast and safe method to foster a personalized antibiotic therapy. Additionally, NAT based analysis of various resistance genes is ideally suited to perform epidemiological studies. This is e.g. useful to react towards changes in the spectrum of resistant bacteria present within individual healthcare institutions.
The ampliCube MDR Panel tests are a highly sensitive and flexible real-time PCR test system for the detection of transmittable resistance genes, either from bacterial culture or directly from swabs.
Test principle and procedure and Evaluation
Evaluation
Performance data of all ampliCube MDR Panel tests were evaluated with the LightCycler® 480 Instrument II (Roche). The following nucleic acid extraction systems were used for the performance assessment: MagNA Pure® Compact (Roche) and the Total Nucleic Isolation Acid Kit I (Roche).
Product Advantages for Your Benefit
- Fast analysis of individual resistance mechanisms foster an optimized antibiotic therapy
- High sensitivity of each MDR panel test
- Individual combination of the single MDR panel test kits allows a personalized resistance analysis
- Wide range of application: The sample material may derive from culture plates with selection medium or directly from a bacterial swab
- Easy to use: enzyme mix is colored blue for visual control during test setup
- Complete Kits: including positive and negative control, internal control (extraction and inhibition control) as well as standards for quantification
- Good compatibility: standard cycling program – harmonized with all further ampliCube tests
- High flexibility: use of different extraction methods and real-time PCR cyclers possible
- CE-IVD label: The ampliCube MDR Panel 1-6 tests meet the high standards of the European Directive 98/79/EC on in vitro diagnostic medical devices
Overview ampliCube MDR Panel 1-6 – Combine our MDR real-time PCR kits according to your requirements!
