
recomLine Yersinia IgG 2.0
recomLine Yersinia IgA [IgM] 2.0
Strip-Immunoassay with antigens produced by recombinant techniques for the detection of IgG, IgM or IgA antibodies directed Y. enterocolitica and Y. pseudotuberculosis. Detection of species-specific IgG antibodies makes it possible to differentiate between Y. enterocolitica and Y. pseudotuberculosis.
The enteropathogenic Yersinia species, Yersinia enterocolitica and Yersinia pseudotuberculosis, have a global distribution and have become increasingly important in recent years. These pathogens are transmitted orally either in food (especially meat) or in contaminated water. Typical symptoms of an acute Y. enterocolitica infection are watery, sometimes bloody diarrhoea with abdominal pain, vomiting and fever. With Y. pseudotuberculosis infection, mesenteric lymphadenitis with terminal ileitis can be observed clinically. Since it is difficult to distinguish this clinical picture from appendicitis, it is also referred to as “pseudoappendicitis”. Post infectious complications such as reactive arthritis, erythema nodosum and other rheumatic diseases can occur, especially with HLA-B27 carriers. High and persistent IgA titres against Yersinia antigens are characteristic of these patients.
For the recomLine Yersinia tests, plasmid-encoded virulence proteins localised on the cell surface (Yersinia outer proteins) and adhesins are produced recombinantly in order to differentiate serologically between the species Y. enterocolitica and Y. pseudotuberculosis. These proteins are only expressed by Yersinia strains that are pathogenic to humans. The recomLine Yersinia tests make it possible to detect past Yersinia infections, and are thus ideally suited for identification of Yersinia-induced immunopathological complications and chronic yersiniosis. Detection of IgG and IgA antibodies can be a very useful diagnostic tool if Yersinia-induced arthritis is suspected.
Product advantages
- Use of recombinant Yersinia antigens
- Identification of all pathogenic Yersinia by means of Yersinia outer proteins (YOPs)
- Serological differentiation of Y. enterocolitica and Y. pseudotuberculosis infections is possible for the first time with the use of new species-specific Yersinia antigens (PsaA, MyfA)
- No cross reactions with Brucella and other pathogens, as well as no interference caused by LPS
- Easy test procedure; automation possible
- Easy and objective evaluation and documentation by recomScan software
- Test procedure and reagents identical in all MIKROGEN strip tests - reagents exchangeable
- Separate detection of IgG, IgM and IgA antibodies - can provide helpful evidence for the clarification of reactive arthritis and other symptoms
- CE label: The recomLine Yersinia tests meet the high standard of the EC directive 98/79/EC on in vitro diagnostic medical devices
Testprinciple and procedure
1st Incubation
A test strip loaded with Yersinia antigens is incubated with diluted serum or plasma in a dish for 1 hour.
wash 3 times
2nd Incubation
Peroxidase conjugated anti-human antibodies (IgG, IgA or IgM specific) are added. Incubate for 45 minutes.
wash 3 times
Color reaction
8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.
Evaluation
Diagnostic Sensitivity
Diagnostic Specificity
Differentiation between Y. enterocolitica and Y. pseudotuberculosis by detecting species-specific IgG antibodies
* Classified as Y. enterocolitica samples with a positive Widal test result
** Classified as Y. pseudotuberculosis samples with a positive culture and PCR results
