CLIA MxA
MxA plays a key role in host defense against viral infection by acting directly on viral particles (RNA, DNA) in the cell and is involved in the activation of the endoplasmic reticulum. Elevated blood MxA levels are due to the presence of viruses in the body or increased concentrations of IFN I (IFN-α and IFN-β). Thus, it is a specific marker of viral infection as a counterpart to PCT in bacterial infections.
Measurement of MxA is suitable for the confirmation of respiratory diseases (Rotavirus, rhinovirus, respiratory syncytial virus, parainfluenza and influenza virus, metapneumovirus), but also in connection with COVID-19.
The use of therapeutic IFN-beta is associated with the development of neutralizing antibodies (NAbs). NAbs inactivate IFN-beta in 5-10 % of patients. Determination of MxA levels is recommended to monitor the effectiveness of treatment with Interferon (IFN-α and IFN-β) in patients with multiple sclerosis, Sjögren's syndrome, and in women with HCV planning to become pregnant. The decrease of the MxA levels indicates the need to modify the therapy used.
Advantages
- Determination of MxA (Myxovirus protein A) concentration in human whole blood samples
- Identification and monitoring upper respiratory virus infection in neonates and infants, and post-surgery patients
- Monitoring of Interferon therapy effectiveness in multiple sclerosis and Sjögren's syndrome patients
- For each CLIA kit a corresponding control set is available
| Assay time | within 30 min. (after lysis) |
| Assay stability | 30 days in-board-stability / in-use-stability until the expiration date at storage temperature 2-8 °C |
| Sample matrix | whole blood and after processing diluted whole blood lysate |
| Sample volume | 30 µL of primary sample |
| Sample stability | min. 24 hours at RT and in the fridge |
| Measuring range | 0,625 – 40 ng/ml, after taking into account the dilution factor = 31.25 - 2000 ng/ml. Dilution factor of primary sample: 50x |
| Assay/kit content | Reagent Cartridge with specific reagents for the assay, magnetic particles, calibrators |
| Note | Lysing buffer non included |