CLIA SS-A
in human serum or plasma
SS-A/Ro antigen is a ribonucleoprotein (RNP) complex localized in the nucleus and cytoplasm. It consists of hYRNA (small cytoplasmic RNA) and two protein antigens of 52 kDa and 60 kDa (Ro52 and Ro60).
Autoantibodies against Ro60 a Ro52 are often found together; anti-SS-B/La antibodies may also occur. Presence of the SS-A/Ro autoantibodies is typical for Sjögren’s syndrome (50 – 70%) and systemic lupus erythematodes (40 – 50%), especially for certain clinical subunits (subacute cutaneous lupus, neonatal lupus), and rheumatoid arthritis. Anti-SS-A/Ro antibodies can be detected approximately 3 years before the SLE diagnosis is reached. SS-A/Ro antibodies are also associated with features of extraglandular inflammation in SLE patients including vasculitis, purpura, cytopenias, and adenopathy.
While anti-Ro60 antibodies are primarily specific for systemic lupus erythematodes and Sjögren’s syndrome, anti-Ro52 antibodies were proved to be individually present (without anti-Ro60) in systemic scleroderma (20%) and myositis (35%), especially in anti-Jo-1 positive patients. Anti-Ro52 as well as anti-SS-B/La antibodies significantly raise the risk of congenital atrioventricular block (CAVB) development. They may be also present in autoimmune liver disease.
Antigen Used: Highly purified native antigen SS-A/Ro60 (60 kDa) and recombinant antigen SS-A/Ro52 (52 kDa).
Advantages
- Detection of anti-SS-A/Ro antibodies, IgG class
- Evaluating patients with signs and symptoms of a connective tissue disease and positive test for antinuclear antibodies
- A positive result for SS-A/Ro antibodies indicates connective tissue disease, including Sjögren’s syndrome, systemic lupus erythematodes (SLE), or rheumatoid arthritis
- Predictive marker of the development of SLE
- Intended for human serum and plasma
- Highly purified native antigen SS-A/Ro60 (60 kDa) and recombinant antigen SS-A/Ro52 (52 kDa) antigens are used
- The kits are CE-IVD certified and intended for professional use
- For each CLIA kit a corresponding control set is available
| Assay stability | 30 days in-board-stability / in-use-stability until the expiration date at storage temperature 2-8 °C |
| Sample matrix | Serum, Plasma |
| Sample volume | 10 µL |
| Measuring range | 0,5-320 U/ml |
| Assay/kit content | Reagent Cartridge with specific reagents for the assay, magnetic particles, calibrators |