recomLine Treponema
against Treponema pallidum in human serum or plasma
Mikrogen recomLine Treponema tests are serological, qualitative line immunoassays, based on recombinantly produced antigens for the detection of IgG or IgM antibodies against Treponema. Treponema pallidum subsp. pallidum is the causative agent of syphilis or lues, a chronic disease unique to humans.
Serological syphilis diagnostics provide for antibody screening with a sensitive screening test (TPHA/TPPA, ELISA) in the first stage. If the result of the screening test is inconclusive or positive, a confirmatory test (FTA-ABS, immunoblot) is performed in the second stage to ensure specificity. Here, the immunoblot plays an important role, since this technique makes it possible to determine antibody formation against essential antigens of Treponema pallidum and at the same time to differentiate the affiliation of the reacting antibodies to the immunoglobulin class IgG or IgM.
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Advantages
- Separate detection of IgG and IgM antibodies
- Additional safety due to the new antigens Tp257 and Tp453 with high specificity for Treponema pallidum
- Easy and clear interpretation due to two-band criterion
- Partial and full automation, software-based evaluation (recomScan) and integration with laboratory information system possible
- Highest sensitivity and specificity through the use of recombinantly produced Treponema-specific antigens:
| Recombinant antigen | Function | Size (kDa) |
| Tp47 | Membrane protein | 47 |
| TmpA | Membrane protein | 42 |
| Tp257 (Gpd) | Glycerophosphodiester phosphodiesterase, membrane protein | 39 |
| Tp453 | Membrane protein | 28 |
| Tp17 | Membrane protein | 17 |
| Tp15 | Membrane protein | 15 |
Safe and reliable
Validity check by integrated, strip-specific cutoff and antibody class controls
Flexible and compatible
Combination of all Mikrogen line immunoassays possible - uniform processing and interchangeable reagents
High Standard
CE mark: The recomLine Treponema tests meet the high standard of the EC Directive 98/79/EC for in vitro diagnostic medical devices.